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KMID : 0364820130490030228
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Korean Journal of Microbiology
2013 Volume.49 No. 3 p.228 ~ p.236
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Genetic Properties and Antimicrobial Resistance of Campylobacter jejuni Isolates from Diarrhea Patients in Gyeonggi-do
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Hur Eun-Seon
Park Po-Hyun
Kim Jong-Hwa
Son Jong-Sung
Yun Hee-Jeong
Lee Yea-Eun
Choi Yun-Sook
Yoon Mi-Hye
Lee Jung-Bok
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Abstract
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Campylobacter jejuni is one of important food-borne pathogens causing human gastroenteritis. We isolated 42
strains of C. jejuni from diarrhea patients and 4 food-poisoning outbreaks in 2010, Gyeonggi-do. In this study, 42
strains were tested for genetic characteristics, the serotype distribution and antimicrobial resistant rate. The presence
of hipO (100%), cdtB (100%), and mutated gyrA (95.2%) genes was detected in C. jejuni by polymerase chain
reaction (PCR). Detection of mutated gyrA gene correlated with ciprofloxacin resistance. Forty isolates had mutated
gyrA gene and were actually resistant to ciprofloxacin. Furthermore, comparing the gyrA DNA sequence data,
ciprofloxacin-resistant isolates had a mutation of the DNA sequence from ACA (threonine) to ATA (isoleucine). But
41 strains (97.6%) of patient isolates were susceptible to erythromycin and azithromycin. A total of 35.7% among 42
C. jejuni isolates were identified into 4 different serotypes. The serotype distribution of C. jejuni strains were shown
to be HS2(B), HS3(C), HS4(D), HS19(O). To investigate the genotypes of C. jejuni isolated in Gyeonggi province,
repetitive sequence polymerase chain reaction (rep-PCR) analysis and SmaI-digested pulsed-filed gel electrophoresis
(PFGE) profile analysis were performed. From the PFGE analysis of 42 C. jejuni strains, 12 clusters of PFGE profile
were obtained. On the other hand, 11 clusters of rep-PCR profile were obtained from 42 strains of C. jejuni.
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KEYWORD
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Campylobacter jejuni, gyrA, antimicrobial susceptibility, PFGE, rep-PCR
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